Protein Molecular Weight from Amino Acid Sequence
Quickly calculate the approximate molecular weight of a protein given its amino acid sequence.
Protein Molecular Weight Calculation
Enter the protein sequence (single-letter amino acid codes).
Use single-letter codes (A, R, N, D, C, E, Q, G, H, I, L, K, M, F, P, S, T, W, Y, V).
About Protein MW from Amino Acid Sequences
The mass of a protein can be estimated by summing the average residue masses of its amino acids. Each amino acid contributes a unique side chain that affects the overall molecular weight. This calculator uses approximate residue masses to provide a rapid estimate in Daltons (g/mol), which is then also expressed in kilodaltons (kDa) for convenience.
In reality, the exact molecular weight can be influenced by post-translational modifications, disulfide bonds, and variations in terminal groups. For precise analysis, techniques like mass spectrometry are recommended.
Protein Molecular Weight estimates are commonly used in experiment design involving protein purification, electrophoresis, or other biochemical assays where mass-dependent parameters may impact the procedure.