Buffer Preparation Calculator
Calculate buffer compositions for your experiments.
Henderson-Hasselbalch Equation
Calculate buffer composition using the Henderson-Hasselbalch equation.
About Buffer Preparation
Buffer preparation ensures that biological or chemical systems remain at a stable pH during experiments. The Henderson-Hasselbalch equation or direct calculations for specific buffers (such as acetate, phosphate, or Tris) are commonly used in labs.
A buffer typically consists of a weak acid (HA) and its conjugate base (A⁻). By adjusting the ratio of acid to base, it is possible to achieve and maintain a target pH even when small amounts of acid or base are added.
Many standard biological assays and protocols rely on precise buffer formulations to ensure consistent performance and accurate results.
Maintaining the correct pH is crucial for enzyme activity, protein stability, and other reactions dependent on proton concentration. Whether using the Henderson-Hasselbalch equation or pre-defined buffer systems, carefully preparing your solutions contributes to reliable experimental outcomes.