Michaelis-Menten Constant (Km) Calculator
Calculate the Michaelis-Menten constant (Km) for enzyme kinetics analysis.
Km Calculation
Enter the rate constants to calculate Km.
About the Michaelis-Menten Constant (Km)
The Michaelis-Menten constant (Km) is a fundamental parameter in enzyme kinetics. It represents the substrate concentration at which the reaction rate is half of its maximum value (Vmax). A lower Km indicates a higher affinity of the enzyme for its substrate.
The formula used in this calculator is:
Km = (k₋₁ + k₂) / k₁
Where:
- k₁ is the rate constant for substrate binding
- k₋₁ is the rate constant for substrate unbinding
- k₂ is the rate constant for product formation