Analyte Estimation using Spectrophotometric Methods & Excel
Beer law or Beer-Lambert’s law states that the absorbance of a solution is proportional to its concentration and pathlength. Mathematically,
Absorbance, A = e C L
And, A = e C L – Equation 1
where,
e = absorptivity at specified wavelength and temperature (M-1cm-1)
L = path length (in cm)
C = molar concentration of the solute